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KMID : 0903520000430020081
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2000 Volume.43 No. 2 p.81 ~ p.85
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Purification and Characterization of Biopolymer by Bacillus coagulans CE-74
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Abstract
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Screening was performed to isolate biopolymer-producing microorganisms from natural sources. The bacteriological characteristics of this strain and physicochemical properties of the biopolymer produced were investigated. The bacterial strain was identified as a Bacillus coagulans. Crude biopolymer treated with ethanol and actone was purified to fraction Ⅰ and Ⅱ by ion exchange chromatography and gel chromatography (Shephadex G-100 and Shepharose CL-2B). Analysis of chemical composition and various color reaction revealed that the polymer is composed of amino acids. It was confirmed that fraction Ⅱ is a homopolymer of glutamic acid and fraction Ⅰ is a homopolymer of lysine by analysis data of amino acid analyzer, GC and IR.
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